Monitoring cell signalling pathways accurately is essential for understanding many cellular processes and disease states. Phosphoproteins play a critical role in these processes and reliable sensitive measurement techniques are important to help further basic research and drug discovery research.
ELISA-ONE™ assay kits have been developed for key intracellular phosphoproteins (see list below), with each assay recognizing a specific phospho-epitope on each target. In addition, ELISA-ONE™ assay kits for each target, independent of phosphorylation status, are available for most targets for normalization.
ELISA-ONE™ assay kits are available as full 96-well assay kits, containing all components necessary for the assay.
ELISA-ONE™ Product List
| Kit Description | Part # | Kit format |
| Test kits for measuring phospho-AKT 1/2/3 (S473) proteins | ELK001 | 96-well kit |
| Test kits for measuring phospho-ERK 1/2 (T202/Y204) proteins | ELK004 | 96-well kit |
| Test kits for measuring phospho-NF-κB p65 (S536) proteins | ELK007 | 96-well kit |
| Test kits for measuring phospho-SMAD 1 (S463/465) proteins | ELK010 | 96-well kit |
| Test kits for measuring phospho-STAT3 (Y705) proteins | ELK013 | 96-well kit |
| Test kits for measuring phospho-STAT5 A/B (Y694/699) proteins | ELK016 | 96-well kit |
| Test kits for measuring Total AKT 1/2/3 proteins | ELK002 | 96-well kit |
| Test kits for measuring Total ERK 1/2 proteins | ELK005 | 96-well kit |
| Test kits for measuring Total NF-κB p65 proteins | ELK008 | 96-well kit |
| Test kits for measuring Total SMAD 1 proteins | ELK011 | 96-well kit |
| Test kits for measuring Total STAT3 proteins | ELK014 | 96-well kit |
| Test kits for measuring Total STAT5 proteins | ELK017 | 96-well kit |
| Test kits for measuring phospho-AKT 1/2/3 (S473) and Total AKT 1/2/3 |
ELK003 | 96-well kit |
| Test kits for measuring phospho-ERK 1/2 (T202/Y204) and Total ERK 1/2 |
ELK006 | 96-well kit |
| Test kits for measuring phospho- NF-κB p65 (S536) and Total NF-κB p65 |
ELK009 | 96-well kit |
| Test kits for measuring phospho-SMAD1 (S463/465) and Total SMAD1 |
ELK012 | 96-well kit |
| Test kits for measuring phospho-STAT3 (Y705) and Total STAT3 |
ELK015 | 96-well kit |
| Test kits for measuring phospho-STAT5 A/B (Y694/699) and Total STAT5 A/B |
ELK018 | 96-well kit |
Additional products for individual sale
| Companion Products | Catalog Number | Description |
| 5 x 96-strip-well microplate | 6300021 | Assay Plate |
| 25 x 96-strip-well microplate | 6300023 | Assay Plate |
| 120 x 96-strip-well microplates | 6300006 | Assay Plate |
| 100 mL 5X Lysis Buffer | EBB003 | 5X Lysis Buffer |
| 100 mL Enhancer Solution | EBB002 | Enhancer Solution |
| 500 mL 10X Wash Buffer | EBB001 | 10X Wash Buffer |