The TF-1 cell line was established from a heparinized bone marrow aspiration sample from a 35 year old male with severe pancytopenia. The cells are completely dependent on IL-3 or GM-CSF for culturing, but in the presence of these factors grows rapidly. TF-1 cells respond to a variety of cytokines, and is a useful tool for studying cytokine signaling mechanisms because they carry a wide range of endogenous receptors. Phosphorylation of endogenous STAT1, STAT3 and STAT5 is readily detectable in these cells, and provides a convenient marker for cytokine response.
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