Surefire – Akt Signaling

ElisaOne AKT 1/2/3 Signaling diagramAKT, also known as protein kinase B (PKB), plays a critical role in cell survival and apoptosis. AKT signaling is particularly important for transducing growth-factor mediated signaling, and regulating apoptotic signaling molecules by phosphorylating and inactivating several pro-apoptoptic proteins, including BAD and caspase-9. Disregulated AKT signaling can result in loss of apoptotic regulation, and so this pathway is a major target of anti-cancer research. Another important function of AKT is the regulation of glycogen synthesis through negative regulation of GSK-3α and β activity by phosphorylation.

TGR has developed several assays for the interrogation of these pathways:

AKT pathway targets

  • phospho-PDK-1phospho-BAD
  • phospho-caspase 9
  • phospho-4EBP1
  • phospho-GSK3α
  • phospho-GSK3β
  • phospho-AKT
  • Total AKT
  • phospho-mTOR
  • phospho-p70S6 kinase

Click here for technical downloads relating to these pathways

Ordering information

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